Nuno Guimarães

Junior Researcher

Research group: Biotechnology

Background: PhD in Chemical and Biological Engineering - University of Minho, Portugal

Major research topics

• Fluorescence in situ Hybridization
• Nucleic Acid Mimics
• Target delivery systems
• Antisense therapeutics

Selected Publications

Santos RS, Lima CC, Carvalho D, Meireles F, Guimarães N, Azevedo NF. Response surface methodology to optimize peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) in Saccharomyces cerevisiae. LWT - Food Science and Technology. 2017;80:27-31. doi: 10.1016/j.lwt.2017.01.077.

Fontenete S, Carvalho D, Guimaraes N, Madureira P, Figueiredo C, Wengel J, et al. Application of locked nucleic acid-based probes in fluorescence in situ hybridization. Appl Microbiol Biotechnol. 2016;100(13):5897-906. doi: 10.1007/s00253-016-7429-4. PubMed PMID: 26969040.

Santos RS, Dakwar GR, Xiong R, Forier K, Remaut K, Stremersch S, et al. Effect of Native Gastric Mucus on in vivo Hybridization Therapies Directed at Helicobacter pylori. Mol Ther Nucleic Acids. 2015;4:e269. doi: 10.1038/mtna.2015.46. PubMed PMID: 26645765.

Guimaraes N, Azevedo NF, Vieira MJ, Figueiredo C. Water-induced modulation of Helicobacter pylori virulence factors. Mem Inst Oswaldo Cruz. 2014;109(4). Epub Jun 2014. doi: 10.1590/0074-0276140024.

Fontenete S, Guimarães N, Leite M, Figueiredo C, Wengel J, Filipe Azevedo N. Hybridization-Based Detection of Helicobacter pylori at Human Body Temperature Using Advanced Locked Nucleic Acid (LNA) Probes. PLoS ONE. 2013;8:e81230. doi: 10.1371/journal.pone.0081230.

Selected Projects

DelNAM - Strengthening the Research Area of Delivery of Nucleic acid mimics (NAMS) into bacteria to fight the antibiotic crisis - WIDESPREAD-05-2017. Duration 2018-2021; Role: Team member.

uFISH - Integração de hibridação in situ fluorescente com microfluídica para deteção rápida de patogéneos alimentares - POCI-01-0145-FEDER-031011. Duration: 2018 - 2021; Role: Team member

ColorISH - Development of a new colorimetric method for the rapid detection of Legionella spp. in water sources - POCI-01-0145-FEDER-29961. Duration: 2018 - 2021Role: Team member

Coded-FISH - Development of a novel color-coded RING FISH method: an innovative tool for the detection of antibiotic resistant clinical pathogens - PTDC/DTP-PIC/4562/2014|16678. Duration: 2016 - 2019; Role: Team member

HELIWATER - Water and associated biofilms as a possible vector for Helicobacter pylori transmission among the human population -  PTDC/BIA-MIC/108811/2008. Duration: 2010 - 2013; Role: Team member